what is the definition of hypothesis in biology

Hypothesis n., plural: hypotheses [/haɪˈpɑːθəsɪs/] Definition: Testable scientific prediction

Table of Contents

What Is Hypothesis?

A scientific hypothesis is a foundational element of the scientific method . It’s a testable statement proposing a potential explanation for natural phenomena. The term hypothesis means “little theory” . A hypothesis is a short statement that can be tested and gives a possible reason for a phenomenon or a possible link between two variables . In the setting of scientific research, a hypothesis is a tentative explanation or statement that can be proven wrong and is used to guide experiments and empirical research.

It is an important part of the scientific method because it gives a basis for planning tests, gathering data, and judging evidence to see if it is true and could help us understand how natural things work. Several hypotheses can be tested in the real world, and the results of careful and systematic observation and analysis can be used to support, reject, or improve them.

Researchers and scientists often use the word hypothesis to refer to this educated guess . These hypotheses are firmly established based on scientific principles and the rigorous testing of new technology and experiments .

For example, in astrophysics, the Big Bang Theory is a working hypothesis that explains the origins of the universe and considers it as a natural phenomenon. It is among the most prominent scientific hypotheses in the field.

“The scientific method: steps, terms, and examples” by Scishow:

Biology definition: A hypothesis  is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess . It’s an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then test it to see if they were right. It’s like a smart guess that helps them learn new things. A scientific hypothesis that has been verified through scientific experiment and research may well be considered a scientific theory .

Etymology: The word “hypothesis” comes from the Greek word “hupothesis,” which means “a basis” or “a supposition.” It combines “hupo” (under) and “thesis” (placing). Synonym:   proposition; assumption; conjecture; postulate Compare:   theory See also: null hypothesis

Characteristics Of Hypothesis

A useful hypothesis must have the following qualities:

• It should never be written as a question.
• You should be able to test it in the real world to see if it’s right or wrong.
• It needs to be clear and exact.
• It should list the factors that will be used to figure out the relationship.
• It should only talk about one thing. You can make a theory in either a descriptive or form of relationship.
• It shouldn’t go against any natural rule that everyone knows is true. Verification will be done well with the tools and methods that are available.
• It should be written in as simple a way as possible so that everyone can understand it.
• It must explain what happened to make an answer necessary.
• It should be testable in a fair amount of time.
• It shouldn’t say different things.

Sources Of Hypothesis

Sources of hypothesis are:

• Patterns of similarity between the phenomenon under investigation and existing hypotheses.
• Insights derived from prior research, concurrent observations, and insights from opposing perspectives.
• The formulations are derived from accepted scientific theories and proposed by researchers.
• In research, it’s essential to consider hypothesis as different subject areas may require various hypotheses (plural form of hypothesis). Researchers also establish a significance level to determine the strength of evidence supporting a hypothesis.
• Individual cognitive processes also contribute to the formation of hypotheses.

One hypothesis is a tentative explanation for an observation or phenomenon. It is based on prior knowledge and understanding of the world, and it can be tested by gathering and analyzing data. Observed facts are the data that are collected to test a hypothesis. They can support or refute the hypothesis.

For example, the hypothesis that “eating more fruits and vegetables will improve your health” can be tested by gathering data on the health of people who eat different amounts of fruits and vegetables. If the people who eat more fruits and vegetables are healthier than those who eat less fruits and vegetables, then the hypothesis is supported.

Hypotheses are essential for scientific inquiry. They help scientists to focus their research, to design experiments, and to interpret their results. They are also essential for the development of scientific theories.

Types Of Hypothesis

In research, you typically encounter two types of hypothesis: the alternative hypothesis (which proposes a relationship between variables) and the null hypothesis (which suggests no relationship).

Simple Hypothesis

It illustrates the association between one dependent variable and one independent variable. For instance, if you consume more vegetables, you will lose weight more quickly. Here, increasing vegetable consumption is the independent variable, while weight loss is the dependent variable.

Complex Hypothesis

It exhibits the relationship between at least two dependent variables and at least two independent variables. Eating more vegetables and fruits results in weight loss, radiant skin, and a decreased risk of numerous diseases, including heart disease.

Directional Hypothesis

It shows that a researcher wants to reach a certain goal. The way the factors are related can also tell us about their nature. For example, four-year-old children who eat well over a time of five years have a higher IQ than children who don’t eat well. This shows what happened and how it happened.

Non-directional Hypothesis

When there is no theory involved, it is used. It is a statement that there is a connection between two variables, but it doesn’t say what that relationship is or which way it goes.

Null Hypothesis

It says something that goes against the theory. It’s a statement that says something is not true, and there is no link between the independent and dependent factors. “H 0 ” represents the null hypothesis.

Associative and Causal Hypothesis

When a change in one variable causes a change in the other variable, this is called the associative hypothesis . The causal hypothesis, on the other hand, says that there is a cause-and-effect relationship between two or more factors.

Examples Of Hypothesis

Examples of simple hypotheses:

• Students who consume breakfast before taking a math test will have a better overall performance than students who do not consume breakfast.
• Students who experience test anxiety before an English examination will get lower scores than students who do not experience test anxiety.
• Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone, is a statement that suggests that drivers who talk on the phone while driving are more likely to make mistakes.

Examples of a complex hypothesis:

• Individuals who consume a lot of sugar and don’t get much exercise are at an increased risk of developing depression.
• Younger people who are routinely exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces, according to a new study.
• Increased levels of air pollution led to higher rates of respiratory illnesses, which in turn resulted in increased costs for healthcare for the affected communities.

Examples of Directional Hypothesis:

• The crop yield will go up a lot if the amount of fertilizer is increased.
• Patients who have surgery and are exposed to more stress will need more time to get better.
• Increasing the frequency of brand advertising on social media will lead to a significant increase in brand awareness among the target audience.

Examples of Non-Directional Hypothesis (or Two-Tailed Hypothesis):

• The test scores of two groups of students are very different from each other.
• There is a link between gender and being happy at work.
• There is a correlation between the amount of caffeine an individual consumes and the speed with which they react.

Examples of a null hypothesis:

• Children who receive a new reading intervention will have scores that are different than students who do not receive the intervention.
• The results of a memory recall test will not reveal any significant gap in performance between children and adults.
• There is not a significant relationship between the number of hours spent playing video games and academic performance.

Examples of Associative Hypothesis:

• There is a link between how many hours you spend studying and how well you do in school.
• Drinking sugary drinks is bad for your health as a whole.
• There is an association between socioeconomic status and access to quality healthcare services in urban neighborhoods.

Functions Of Hypothesis

The research issue can be understood better with the help of a hypothesis, which is why developing one is crucial. The following are some of the specific roles that a hypothesis plays: (Rashid, Apr 20, 2022)

• A hypothesis gives a study a point of concentration. It enlightens us as to the specific characteristics of a study subject we need to look into.
• It instructs us on what data to acquire as well as what data we should not collect, giving the study a focal point .
• The development of a hypothesis improves objectivity since it enables the establishment of a focal point.
• A hypothesis makes it possible for us to contribute to the development of the theory. Because of this, we are in a position to definitively determine what is true and what is untrue .

How will Hypothesis help in the Scientific Method?

• The scientific method begins with observation and inquiry about the natural world when formulating research questions. Researchers can refine their observations and queries into specific, testable research questions with the aid of hypothesis. They provide an investigation with a focused starting point.
• Hypothesis generate specific predictions regarding the expected outcomes of experiments or observations. These forecasts are founded on the researcher’s current knowledge of the subject. They elucidate what researchers anticipate observing if the hypothesis is true.
• Hypothesis direct the design of experiments and data collection techniques. Researchers can use them to determine which variables to measure or manipulate, which data to obtain, and how to conduct systematic and controlled research.
• Following the formulation of a hypothesis and the design of an experiment, researchers collect data through observation, measurement, or experimentation. The collected data is used to verify the hypothesis’s predictions.
• Hypothesis establish the criteria for evaluating experiment results. The observed data are compared to the predictions generated by the hypothesis. This analysis helps determine whether empirical evidence supports or refutes the hypothesis.
• The results of experiments or observations are used to derive conclusions regarding the hypothesis. If the data support the predictions, then the hypothesis is supported. If this is not the case, the hypothesis may be revised or rejected, leading to the formulation of new queries and hypothesis.
• The scientific approach is iterative, resulting in new hypothesis and research issues from previous trials. This cycle of hypothesis generation, testing, and refining drives scientific progress.

Importance Of Hypothesis

• Hypothesis are testable statements that enable scientists to determine if their predictions are accurate. This assessment is essential to the scientific method, which is based on empirical evidence.
• Hypothesis serve as the foundation for designing experiments or data collection techniques. They can be used by researchers to develop protocols and procedures that will produce meaningful results.
• Hypothesis hold scientists accountable for their assertions. They establish expectations for what the research should reveal and enable others to assess the validity of the findings.
• Hypothesis aid in identifying the most important variables of a study. The variables can then be measured, manipulated, or analyzed to determine their relationships.
• Hypothesis assist researchers in allocating their resources efficiently. They ensure that time, money, and effort are spent investigating specific concerns, as opposed to exploring random concepts.
• Testing hypothesis contribute to the scientific body of knowledge. Whether or not a hypothesis is supported, the results contribute to our understanding of a phenomenon.
• Hypothesis can result in the creation of theories. When supported by substantive evidence, hypothesis can serve as the foundation for larger theoretical frameworks that explain complex phenomena.
• Beyond scientific research, hypothesis play a role in the solution of problems in a variety of domains. They enable professionals to make educated assumptions about the causes of problems and to devise solutions.

Research Hypotheses: Did you know that a hypothesis refers to an educated guess or prediction about the outcome of a research study?

It’s like a roadmap guiding researchers towards their destination of knowledge. Just like a compass points north, a well-crafted hypothesis points the way to valuable discoveries in the world of science and inquiry.

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Further reading.

• RNA-DNA World Hypothesis
• BYJU’S. (2023). Hypothesis. Retrieved 01 Septermber 2023, from https://byjus.com/physics/hypothesis/#sources-of-hypothesis
• Collegedunia. (2023). Hypothesis. Retrieved 1 September 2023, from https://collegedunia.com/exams/hypothesis-science-articleid-7026#d
• Hussain, D. J. (2022). Hypothesis. Retrieved 01 September 2023, from https://mmhapu.ac.in/doc/eContent/Management/JamesHusain/Research%20Hypothesis%20-Meaning,%20Nature%20&%20Importance-Characteristics%20of%20Good%20%20Hypothesis%20Sem2.pdf
• Media, D. (2023). Hypothesis in the Scientific Method. Retrieved 01 September 2023, from https://www.verywellmind.com/what-is-a-hypothesis-2795239#toc-hypotheses-examples
• Rashid, M. H. A. (Apr 20, 2022). Research Methodology. Retrieved 01 September 2023, from https://limbd.org/hypothesis-definitions-functions-characteristics-types-errors-the-process-of-testing-a-hypothesis-hypotheses-in-qualitative-research/#:~:text=Functions%20of%20a%20Hypothesis%3A&text=Specifically%2C%20a%20hypothesis%20serves%20the,providing%20focus%20to%20the%20study.

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Last updated on September 8th, 2023

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What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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Scientific Method

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The scientific method is a series of steps followed by scientific investigators to answer specific questions about the natural world. It involves making observations, formulating a hypothesis , and conducting scientific experiments . Scientific inquiry starts with an observation followed by the formulation of a question about what has been observed. The steps of the scientific method are as follows:

Observation

The first step of the scientific method involves making an observation about something that interests you. This is very important if you are doing a science project because you want your project to be focused on something that will hold your attention. Your observation can be on anything from plant movement to animal behavior, as long as it is something you really want to know more about.​ This is where you come up with the idea for your science project.

Once you've made your observation, you must formulate a question about what you have observed. Your question should tell what it is that you are trying to discover or accomplish in your experiment. When stating your question you should be as specific as possible.​ For example, if you are doing a project on plants , you may want to know how plants interact with microbes. Your question may be: Do plant spices inhibit bacterial growth ?

The hypothesis is a key component of the scientific process. A hypothesis is an idea that is suggested as an explanation for a natural event, a particular experience, or a specific condition that can be tested through definable experimentation. It states the purpose of your experiment, the variables used, and the predicted outcome of your experiment. It is important to note that a hypothesis must be testable. That means that you should be able to test your hypothesis through experimentation .​ Your hypothesis must either be supported or falsified by your experiment. An example of a good hypothesis is: If there is a relation between listening to music and heart rate, then listening to music will cause a person's resting heart rate to either increase or decrease.

Once you've developed a hypothesis, you must design and conduct an experiment that will test it. You should develop a procedure that states very clearly how you plan to conduct your experiment. It is important that you include and identify a controlled variable or dependent variable in your procedure. Controls allow us to test a single variable in an experiment because they are unchanged. We can then make observations and comparisons between our controls and our independent variables (things that change in the experiment) to develop an accurate conclusion.​

The results are where you report what happened in the experiment. That includes detailing all observations and data made during your experiment. Most people find it easier to visualize the data by charting or graphing the information.​

The final step of the scientific method is developing a conclusion. This is where all of the results from the experiment are analyzed and a determination is reached about the hypothesis. Did the experiment support or reject your hypothesis? If your hypothesis was supported, great. If not, repeat the experiment or think of ways to improve your procedure.

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hypothesis , something supposed or taken for granted, with the object of following out its consequences (Greek hypothesis , “a putting under,” the Latin equivalent being suppositio ).

In planning a course of action, one may consider various alternatives , working out each in detail. Although the word hypothesis is not typically used in this case, the procedure is virtually the same as that of an investigator of crime considering various suspects. Different methods may be used for deciding what the various alternatives may be, but what is fundamental is the consideration of a supposal as if it were true, without actually accepting it as true. One of the earliest uses of the word in this sense was in geometry . It is described by Plato in the Meno .

The most important modern use of a hypothesis is in relation to scientific investigation . A scientist is not merely concerned to accumulate such facts as can be discovered by observation: linkages must be discovered to connect those facts. An initial puzzle or problem provides the impetus , but clues must be used to ascertain which facts will help yield a solution. The best guide is a tentative hypothesis, which fits within the existing body of doctrine. It is so framed that, with its help, deductions can be made that under certain factual conditions (“initial conditions”) certain other facts would be found if the hypothesis were correct.

The concepts involved in the hypothesis need not themselves refer to observable objects. However, the initial conditions should be able to be observed or to be produced experimentally, and the deduced facts should be able to be observed. William Harvey ’s research on circulation in animals demonstrates how greatly experimental observation can be helped by a fruitful hypothesis. While a hypothesis can be partially confirmed by showing that what is deduced from it with certain initial conditions is actually found under those conditions, it cannot be completely proved in this way. What would have to be shown is that no other hypothesis would serve. Hence, in assessing the soundness of a hypothesis, stress is laid on the range and variety of facts that can be brought under its scope. Again, it is important that it should be capable of being linked systematically with hypotheses which have been found fertile in other fields.

If the predictions derived from the hypothesis are not found to be true, the hypothesis may have to be given up or modified. The fault may lie, however, in some other principle forming part of the body of accepted doctrine which has been utilized in deducing consequences from the hypothesis. It may also lie in the fact that other conditions, hitherto unobserved, are present beside the initial conditions, affecting the result. Thus the hypothesis may be kept, pending further examination of facts or some remodeling of principles. A good illustration of this is to be found in the history of the corpuscular and the undulatory hypotheses about light .